Medical Imaging Convention [rescheduled] Mar 09, 2021 - Mar 10, 2021 — National Exhibition Centre, Birmingham, England
9th SINAPSE Neuro-oncology Imaging Meeting [rescheduled] Mar 11, 2021 09:30 AM - 03:30 PM — West Park Conferencing & Events, 319 Perth Road, Dundee DD2 1NN
Total Body PET 2020 conference [rescheduled] Jun 05, 2021 - Jun 07, 2021 — McEwan Hall, University of Edinburgh

eLearning

SINAPSE experts from around Scotland have developed ten online modules designed to explain medical imaging. They are freely available and are intended for non-specialists.


Edinburgh Imaging Academy at the University of Edinburgh offers the following online programmes through a virtual learning environment:

Neuroimaging for Research MSc/Dip/Cert

Imaging MSc/Dip/Cert

PET-MR Principles & Applications Cert

Applied Medical Image Analysis Cert

Online Short Courses

Crystal structure and mechanism of a bacterial fluorinating enzyme

Author(s): C. J. Dong, F. L. Huang, H. Deng, C. Schaffrath, J. B. Spencer, D. O'Hagan, J. H. Naismith

Abstract:
Fluorine is the thirteenth most abundant element in the earth's crust, but fluoride concentrations in surface water are low and fluorinated metabolites are extremely rare(1,2). The fluoride ion is a potent nucleophile in its desolvated state, but is tightly hydrated in water and effectively inert. Low availability and a lack of chemical reactivity have largely excluded fluoride from biochemistry: in particular, fluorine's high redox potential precludes the haloperoxidase-type mechanism(3,4) used in the metabolic incorporation of chloride and bromide ions. But fluorinated chemicals are growing in industrial importance, with applications in pharmaceuticals, agrochemicals and materials products(5-7). Reactive fluorination reagents requiring specialist process technologies are needed in industry and, although biological catalysts for these processes are highly sought after, only one enzyme that can convert fluoride to organic fluorine has been described(8). Streptomyces cattleya can form carbon-fluorine bonds(9) and must therefore have evolved an enzyme able to overcome the chemical challenges of using aqueous fluoride. Here we report the sequence and three-dimensional structure of the first native fluorination enzyme, 5'-fluoro-5'-deoxyadenosine synthase, from this organism. Both substrate and products have been observed bound to the enzyme, enabling us to propose a nucleophilic substitution mechanism for this biological fluorination reaction.

Full version: Available here

Click the link to go to an external website with the full version of the paper


ISBN: 0028-0836
Publication Year: 2004
Periodical: Nature
Periodical Number: 6974
Volume: 427
Pages: 561-565
Author Address: