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eLearning

SINAPSE experts from around Scotland have developed ten online modules designed to explain medical imaging. They are freely available and are intended for non-specialists.


Edinburgh Imaging Academy at the University of Edinburgh offers the following online programmes through a virtual learning environment:

Neuroimaging for Research MSc/Dip/Cert

Imaging MSc/Dip/Cert

PET-MR Principles & Applications Cert

Applied Medical Image Analysis Cert

Online Short Courses

Studying synapses in human brain with array tomography and electron microscopy

Author(s): K. R. Kay, C. Smith, A. K. Wright, A. Serrano-Pozo, A. M. Pooler, R. Koffie, M. E. Bastin, T. H. Bak, S. Abrahams, K. J. Kopeikina, D. McGuone, M. P. Frosch, T. H. Gillingwater, B. T. Hyman, T. L. Spires-Jones

Abstract:
Postmortem studies of synapses in human brain are problematic because of the axial resolution limit of light microscopy and the difficulty in preserving and analyzing ultrastructure with electron microscopy (EM). Array tomography (AT) overcomes these problems by embedding autopsy tissue in resin and cutting ribbons of ultrathin serial sections. Ribbons are imaged with immunofluorescence, allowing high-throughput imaging of tens of thousands of synapses to assess synapse density and protein composition. The protocol takes ~3 d per case, excluding image analysis, which is done at the end of the study. Parallel processing for transmission electron microscopy (TEM) using a protocol modified to preserve the structure in human samples allows complementary ultrastructural studies. Incorporation of AT and TEM into brain banking is a potent way of phenotyping synapses in well-characterized clinical cohorts in order to develop clinicopathological correlations at the synapse level. This will be important for research in neurodegenerative disease, developmental disease and psychiatric illness.

Full version: Available here

Click the link to go to an external website with the full version of the paper


ISBN: 1750-2799 (Electronic)1750-2799 (Linking)
Publication Year: 2013
Periodical: Nat Protoc
Periodical Number: 7
Volume: 8
Pages: 1366-80
Author Address: Massachusetts General Hospital and Harvard Medical School, MassGeneral Institute for Neurodegenerative Disease, Charlestown, MA, USA.