Author(s)

L. Martarello, C. Schaffrath, H. Deng, A. D. Gee, A. Lockhart, D. O'Hagan

ISBN

0362-4803

Publication year

2003

Periodical

Journal of Labelled Compounds & Radiopharmaceuticals

Periodical Number

13

Volume

46

Pages

1181-1189

Author Address

Full version

The use of the key enzyme involved in carbon-fluorine bond formation in Streptomyces cattleya catalysing the formation of 5′-fluoro-5′-deoxyadenosine (5′-FDA) from fluoride ion and S-adenosyl-L-methionine (SAM) was explored for its potential application in fluorine-18 labelling of the adenosine derivative. Enzymatic radiolabelling of [F-18]-5′-FDA was successfully carried out starting from SAM and [F-18]HF when the concentration of the enzyme preparation was increased from sub-mg/ml values to mg/ml values. The purity of the enzyme had no measurable effect on the radiochemical yield of the reaction and the radiochemical purity of [F-18]-5′-FDA. Copyright (C) 2003 John Wiley Sons, Ltd.