Author(s)

K. K. J. Chan, S. Thompson, D. O'Hagan

ISBN

1439-4227

Publication year

2013

Periodical

Chembiochem

Periodical Number

6

Volume

14

Pages

675-677

Author Address

O'Hagan, D Univ St Andrews, EastChem Sch Chem, Biomol Sci Res Complex, St Andrews KY16 9ST, Fife, Scotland Univ St Andrews, EastChem Sch Chem, St Andrews KY16 9ST, Fife, Scotland

Full version

All about Me: Pierre and co-workers have revealed mechanistic details of a tryptophan methyltransferase (TsrM) involved in the biosynthesis of the thiopeptide antibiotic, thiostrepton. Utilising cobalamin and a [4Fe–4S] cluster to generate 2-methyltryptophan from tryptophan, a key difference between this enzyme and other radical SAM methyltransferases is that the reaction is not initiated by a single-electron reduction of SAM to generate 5′-dA⋅.